Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | lower effect on P-beta-gal II than P-beta-gal I | Lactobacillus gasseri | |
Fe2+ | 60% increase of activity | Lactobacillus gasseri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-Glucose-6-phosphate | 63% of activity | Lactobacillus gasseri | |
iodoacetamide | - |
Lactobacillus gasseri | |
N-ethylmaleimide | - |
Lactobacillus gasseri | |
p-chloromercuribenzoic acid | - |
Lactobacillus gasseri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.76 | 0.8 | o-nitrophenyl-beta-D-galactopyranoside 6-phosphate | - |
Lactobacillus gasseri |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 78% of activity at 1 mM CaCl2 | Lactobacillus gasseri | |
Hg2+ | complete inhibition at 1 mM HgCl2 | Lactobacillus gasseri | |
Mg2+ | slight inhibition at 1 mM MgCl2 | Lactobacillus gasseri | |
Mn2+ | slight inhibition at 1 mM MnCl2 | Lactobacillus gasseri | |
Zn2+ | 98.4% inhibition at 1 mM ZnSO4 | Lactobacillus gasseri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose-6-phosphate + H2O | Lactobacillus gasseri | - |
D-glucose + beta-D-galactose-6-phosphate | - |
? | |
lactose-6-phosphate + H2O | Lactobacillus gasseri JCM 1031 | - |
D-glucose + beta-D-galactose-6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus gasseri | - |
JCM 1031 | - |
Lactobacillus gasseri JCM 1031 | - |
JCM 1031 | - |
Purification (Comment) | Organism |
---|---|
- |
Lactobacillus gasseri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose-6-phosphate + H2O | - |
Lactobacillus gasseri | D-glucose + beta-D-galactose-6-phosphate | - |
? | |
lactose-6-phosphate + H2O | - |
Lactobacillus gasseri JCM 1031 | D-glucose + beta-D-galactose-6-phosphate | - |
? | |
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O | - |
Lactobacillus gasseri | o-nitrophenol + beta-D-galactose-6-phosphate | - |
? | |
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O | - |
Lactobacillus gasseri JCM 1031 | o-nitrophenol + beta-D-galactose-6-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
P-beta-gal | I and II | Lactobacillus gasseri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
P-beta-gal II | Lactobacillus gasseri |
50 | - |
P-beta-gal I | Lactobacillus gasseri |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | P-beta-gal II, stable below 40°C, inactivated at 60°C | Lactobacillus gasseri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 5.5 | - |
Lactobacillus gasseri |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | - |
Lactobacillus gasseri |